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Fig. 5 | BMC Biology

Fig. 5

From: Systematic prediction of degrons and E3 ubiquitin ligase binding via deep learning

Fig. 5

E3-degron interactions impact half-lives of substrates. a Distribution of half-lives for proteins with different densities of degrons in four non-dividing cell types. The half-lives used were replicate 1 of four cell types in original paper [39], and the results of replicate 2 were identical (data not shown). Kernel density estimate plot showing the distribution of log10(half-life +‚ÄČ1). b Distribution of half-lives of predicted substrates for each E3. The minimal half-lives in eight experiments of four non-dividing cell types (two replicates per cell type) [39] for substrates were used, and E3s were sorted by the median half-lives of their predicted substrates. c Half-life experiments of mutated and wild-type CBX6, the experiment is repeated once (Additional file 5: Table S4). d Co-immunoprecipitation experiment between mutated and wild-type CBX6 with SPOP, the experiment is repeated once (Additional file 5: Table S4). Cell lysates were incubated with anti-HA agarose beads, and the immunoprecipitates were analyzed by Western Blot with anti-HA and anti-SPOP antibodies

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