Fig. 2

Mitochondria and myogenesis-related gene and protein levels in response to Tug1 knockdown during myocyte differentiation, in vitro. A C2C12 myoblasts were transfected with Tug1 or control LNAs (25 nM) for 24 h during proliferation (Prolif) or for 1 to 4 days during differentiation (Diff). B Quantification of myotube diameter and representative phase-contrast images. Data are measurements of ≥20 individual myotubes per well from n=3 replicate wells, analysed by two-way ANOVA for main effects of LNA and time, with significant interactions analysed by Bonferroni post hoc tests: **p < 0.01 Tug1 KD compared to control. C Gene expression of selected targets by RT-qPCR, n=6 replicates per group and time point. D Representative immunoblots of protein expression in control (C) and Tug1 KD (T) samples E quantified relative to total protein (stain free); data are mean (SD) for n=6 (triplicate wells across two independent experiments), analysed by two-way ANOVA for main effects of LNA and time, with significant interactions analysed by Bonferroni post hoc tests: *p < 0.05, **p < 0.01, ***p < 0.001 Tug1 KD compared to control. See also Additional file 1: Supplementary Fig. S2