Fig. 1

RNF133 is a testis-specific E3 ubiquitin ligase. A (Left and right), multi-tissue RT-PCR expression analysis of Zswim2, Rnf133, Rnf148, and Rnf151 in mice and humans, respectively. Hprt and GAPDH were used as expression controls. (Middle) RT-PCR from mouse testes at various postnatal days. Hprt was used as an expression control. B Protein domain structures of mouse RNF133 protein (top) and mouse RNF148 protein (bottom). C Sequence alignment of proteins of mouse RNF133 and RNF148 (top), and human RNF133 and RNF148 (bottom). Identified amino acids are highlighted in dark blue. The green and red underbars indicate the transmembrane region and the RING finger domain, respectively. D Genomic structure and strategy of generating KO of mouse Rnf133, and the genetic sequences deleted by the CRISPR/Cas9 system. E Genotyping of Rnf133 alleles. Primers shown in D amplify specific amplicons for the WT (Fw-Rv1) or KO (Fw-Rv2) allele