Fig. 6
From: The testis-specific E3 ubiquitin ligase RNF133 is required for fecundity in mice
RNF133 interacts with UBE2J1. A Western blot analysis of immunoprecipitation with exogenous mouse/human RNF133-FLAG and HA-UBE2J1, human UBE2C, human RNF151, and human RNF133-mutant-FLAG in HEK293 cells. The anti-HA antibody was used for immunoprecipitation and the anti-FLAG antibody was used for Western blot analysis. This experiment was replicated three times and representative blots are presented. B Immunofluorescent staining with exogenous mouse/human RNF133-FLAG and HA-UBE2J1 in HEK293 cells. The anti-FLAG antibody and the anti-HA antibody were used for RNF133 (red) and UBE2J1 (green), respectively. Scale bar, 10 µm. This experiment was replicated three times and representative images are presented