Fig. 7
From: Kinesin-3 motors are fine-tuned at the molecular level to endow distinct mechanical outputs
Positively charged lysine resides in the K-loop influence microtubule bending in cells. COS-7 cells were cotransfected with plasmids coding for mCherry-α-tubulin and individual members of constitutively active alanine (All-ala) mutants of kinesin-3 A KIF1A(1-393LZ), B KIF13A(1-411ΔP), C KIF13B(1-412ΔP), and D KIF16B(1–400), and kinesn-1 E KHC(1–560), in which positively charged lysine residues in the K-loop were mutated to alanine as described previously [17]. Yellow dotted line indicates the cell boundary, and asterisk indicates the nucleus. Scale bars, 10 μm