Fig. 5
From: Segmentation, tracking and cell cycle analysis of live-cell imaging data with Cell-ACDC
Quantitative analysis of Htb1-mCitrine expression as a function of cell cycle and cell volume. A Total amount of Htb1-mCitrine (total cellular fluorescence intensity after background subtraction) as a function of time for the first cell cycle of new-born daughter cells (n=48) and older cells (n=89). Single cell traces are aligned at bud emergence (time = 0). B Amount of Htb1-mCitrine as a function of cell volume at birth (blue) and directly before cytokinesis (combined signal of mother and bud, orange). Signal from untagged strains used as autofluorescence (Af) control is negligible. Results from A and B are consistent with our previous analysis [35]. C Concentration of Htb1-mCitrine retained in mother cells at cell division, shown as a function of division number (boxplot whiskers: 1.5 IQR)