Fig. 1

Perturbation of H3K27me3 levels in Hmga2 wt and KO cells upon the induction of EpiLCs. A Western blotting images showing the levels of histone modifications (H3K27me3, H3K4me3, H3K9me2, H3K9me3, and H3K27ac) in wt and KO PSCs at day 0 (undifferentiated cells) and at three different times after the induction of the transition into EpiLCs (day 1, day 2, and day 3). The levels of the histone H3 were used as control. Two wt and two KO clones were used. Quantification and statistical analysis are reported in Additional file 1: Fig. S1B. B Western blotting and relative quantification graphs showing the levels of H3K27me3, H3K27me2, and H3K27ac during EpiLC establishment (time course experiments: days 1, 2, and 3) of wt and KO cells. C Western blotting images and relative quantification graph showing the levels of H3K27me3 in wt and KO PSCs during SFEB differentiation. D Western blotting images and relative quantification graph showing the levels of the PRC2 proteins Suz12 and Ezh2 during EpiLC establishment (time course experiments: days 1, 2, and 3) of wt and KO cells. The data in the graphs represent the mean ± SD (n=3 biological replicates) of H3K27me3, H3K27me2, and H3K27ac against total H3 (panels B and C) or of Suz12 and Ezh2 signal intensity against Gapdh expressed as arbitrary units (a.u.). Student’s t-test, two tailed (ns: not significant, *p<0.05, **p<0.01)