Fig. 4

Hmga2 silencing induced a phenotype identical to that observed in Hmga2 KO cells. A Lmnb1 (red) and Emerin (green) immunofluorescence on Hmga2 wt and KO cells (day 1 of EpiLC induction) showing the nuclear abnormalities typical of Hmga2 KO cells (single z-plane, ROI 1024×1024, scale bar= 50μm). Quantification graphs obtained by counting >150 nuclei/condition. B Lmnb1 (red) and Emerin (green) immunofluorescence at day 3 after the induction of EpiLCs upon Hmga2 silencing. Nuclear blebbing and enlarged nuclei were surrounded by both Lmnb1 and Emerin. DAPI (blue) was used to counterstain the nuclei. A single plane of the z-stack projection is shown. Scale bar= 50 μm. Quantification graphs obtained by counting >300 nuclei/condition. Error bars represent standard deviation. Statistical significance on three biological replicate experiments was determined using Student’s t-test (***p<0.001)