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Fig. 3 | BMC Biology

Fig. 3

From: Griottes: a generalist tool for network generation from segmented tissue images

Fig. 3

a Sections of a MSC spheroid imaged with a light-sheet microscope. The technique allows in-depth imaging of tissue structures. Scale bar is 50 μm. b 3D network representation of a MSC spheroid. Different cell types are identified based upon CD146 fluorescence measurements. c Comparison between the degree distribution of an example spheroid (panel b, bars) and the batch distribution (N = 5, red line). d Comparison between the link-length distribution of an example spheroid (bars) and the batch distribution (red line). e The network representation makes it possible to identify cells on the outer layer of the spheroid (red) from the inner cells (blue). We can “peel off” the outer layers successively, revealing the inner structure and composition of the spheroid. f Cell degree as a function of the layer number, the average degree is larger for the layers near the center of the spheroid. Blue dots show one example spheroid and red dashed line represents average over the experimental batch. g Distance between cell centers (in μm) as a function of the layer number. Blue dots show one example spheroid and red dashed line represents average over the experimental batch

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