Fig. 5

Conserved epigenetic modifiers and histone modifications are present at different levels in octopus tissues. A Phylogenetic tree of SETD1B, responsible for H3K4me3 deposition, in a representative subset of 19 metazoan and outgroup species. Colors indicate phyla (blue = Chordata; pink = Mollusca; orange = Porifera), and octopus is indicated with an icon. B Phylogenetic tree of SETDB1, responsible for H3K9me3 deposition, in a representative subset of 19 metazoan and outgroup species. Colors indicate phyla (blue = Chordata; pink = Mollusca; orange = Porifera), and octopus is indicated with an icon. C Heatmap of the main histone methylation factors, methyltransferase (writers), and demethylases (erasers). D Western blot of histone H3 and relative modification (H3K4me3, H3K9me3, H3K27me3) performed on arm (1.5 cm of Right Arm 2), brain (optical lobe), gills, and hatchling of O. bimaculoides, in comparison with 5 dpf zebrafish larvae. Quantification of each sample measured by Western blot was normalized to histone H3. Each dot represents one biological replicate, except for hatchling where each dot represents a technical replicate of protein isolated from the same biological sample. Bars represent the mean among replicates, and error bars represent standard deviations