Fig. 2
From: Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity
Promoters and terminators influence heterologous gene expression independently and irrespective of the CDS. YFP fluorescence normalized to mCherry at time point 3 h (means ± S.D.; n = 3 replicate cultures, except for all strains with BE2-YFP, where n = 4), with red points depicting the individual replicates (see Additional file 2 for numerical data). Strain numbers are given below the bars. Data was partly replotted in the individual panels. A Effect of the different promoters exemplified on three CDS-YFP fusions. Within each CDS group, only the promoter varies. n.d., not determined. B Effect of the different terminators exemplified on SS3-YFP (left) and SS4-YFP (right). Within each strain group, only the terminator varies. C Range of expression of SS3-YFP (left) and BE2-YFP (right) obtained by varying the promoters and terminators. Promoter/terminator identities are indicated by the shades shown in panels A and B, respectively (the promoter marked with an asterisk is pCUP1). Dark blue bars correspond to the promoter/terminator combinations that were later used to express untagged SS3 and BE2 at strong, medium, or weak levels, with numbers showing the observed fold changes of fluorescence