Fig. 3

Influence of the promoters and terminators on expression levels. Mean YFP fluorescence normalized to mCherry (time point 3 h) of strains that are isogenic except for the promoter (A–C) and terminator (D–G) of the CDS-YFP constructs, respectively. The mean YFP/mCherry fluorescence of one strain is plotted on the x-axis and that of the other on the y-axis. The respective YFP protein fusion is indicated by color. Only promoter/terminator comparisons for which at least four comparisons were available are shown. Solid black lines show linear regressions, in which single outliers from SS1-YFP (C, G) or SS2-YFP (B) fusions were excluded; the corresponding data points are beyond the displayed areas. Dashed gray lines (R2) show regressions that include the outlier samples. Details and statistics are given in Additional file 1: Table S2, and numerical data is given in Additional file 2. R², coefficient of determination