Fig. 5

Maximum likelihood phylogenetic tree of NOX enzyme family. Unrooted tree displaying NOX subfamilies; NOX1-3 (blue circle), NOX4 (red circle), NOX5 (yellow circle), Duox (purple) and RBOH (green circle). Grey circles indicate subfamilies absent within metazoans. Including subunit-regulated NOXA/B and calcium-regulated NOXC/D. Dashed line surrounds sequences of novel calcium-regulated NOX encoded by species of class Demospongiae, named DspNOX. Black numbers on branches indicate bootstrap support. Branch lengths represent evolutionary distances, indicated by the tree scale. Tree displayed using 5 equal-daylight algorithm iterations to improve branch visibility. Constructed based on edited alignment, 1000 bb and the Dayhoff + F + R6 evolutionary model