Fig. 10

osi mutants have a progresive shift in LD size. A, C, E Downregulation of osi changes the distribution of lipid droplet size. The total area covered by lipid droplets (reactive area) is not significantly different among genotypes; in the mutant, there is a shift towards smaller lipid droplets. Confocal microscopy images showing lipid staining in fat body cells of controls (+/+), osi^+/− (+/−) and osi^100B/100B (^100B/100B) stained with BODIPY (golden lipid droplets) for 24h (A), 48h (B), and 72h (C) AEL. B, D, F Percentage of area covered by fluorescence or “reactive area” for lipid droplets of animals of 24 (B), 48 (D), and 72 (F) hours AEL. Each dot represents the mean area of each fat body analyzed. The total number of fat bodies per condition is indicated. G, H, I Frequency distribution of lipid droplet size from animals of 24 (G), 48 (H), and 72 (I) hours AEL. Only controls (+/+) and osi^100B/100B are shown to facilitate direct comparison. J, K, L Lipid droplet roundness measured by ImageJ as a way of assess structural integrity for 24h (J), 48h (K), and 72h (L) AEL. All panels: Shapiro-Wilk test was used for normality assessment; when normality was confirmed, a one-way ANOVA with Bonferroni’s multiple comparisons was performed. For nonparametric assessment, Kruskal-Wallis with Dunn’s multiple comparisons test were performed (see Additional file 2). Different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. Scale bars represent 10 μm. The total number of samples analyzed is mentioned below in the corresponding column. All datasets and statistical analysis on which the conclusions are based are included in Additional file 2