Fig. 2

P[UAS]^100B is inserted in CG6115 and encodes a Complex I LYR domain-containing protein. A Schematic representation of the insertion locus for P[UAS]^100B and its nearest gene (tweek), indicating the target site of osi^RNAi in relation to the ORF in its mRNA, and the insertion site of the CRIMIC cassette containing Gal4 characteristic of osi^Gal4. B Sequence analysis retrieved a series of different osi orthologs containing a Complex I_ LYR motif. C osi displays high levels of identity and similarity with genes present in the most common model species. D Multiple sequence alignment colored by conservation. Conserved hydrophobic residues are marked in green; conserved positive residues are marked in red. Bottom: histogram representing conserved residue frequencies. This image was generated by Jalview2 (doi:10.1093/bioinformatics/btp033). E qRT-PCR analysis at 72h shows that homozygosity for the P[UAS]^100B insertion reduced osi mRNA levels to about one third relative to control or heterozygous larvae. F qRT-PCR analysis at 72h AEL shows that homozygous P[UAS]^100B insertion did not affect tweek mRNA levels. For experiments shown in E and F, the number of independent observations is included under each data set; each replica included 30 larvae pooled together. A Shapiro-Wilk test was used for normality assessment, and when normality was confirmed, one-way ANOVA with Bonferroni’s multiple comparisons was performed. For nonparametric assessment, Kruskal-Wallis with Dunn’s multiple comparisons test was performed (see Additional file 2). Different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. The total number of analyzed samples is included in the corresponding panel (bottom). All datasets and statistical analysis on which the conclusions are based are included in Additional file 2