Fig. 6
From: orsai, the Drosophila homolog of human ETFRF1, links lipid catabolism to growth control
Expression of human ETFRF1/LYRm5 rescues lethality associated with osi downregulation in the fat body. A qPCR analysis on RNA extracted from whole larvae shows efficient reduction of osi levels when osiRNAi is expressed under pplG4 at 96h AEL. The total number of replicas/genotype is included under each data set; each dataset consisted of 30 larvae pooled together. A two-tailed T-test was used to analyze statistical significance. B Survival curve for pplG4> osiRNAi, pplG4> LYRm5;osiRNAi, pplG4> LYRm5 and +/ LYRm5;osiRNAi genotypes. Animals were surveyed up to 10 days AEL, when all living individuals had arrived to the pupal stage. Survival indicates the percentage of living individuals. The experiment was repeated twice, a representative experiment is shown. C, E Percentage of the population observed outside of the yeast patch. D, F Percentage of clear gut larvae. Larvae were monitored at 72h (C, D) and 96h AEL (E, F). Each dot represents the percentage per experiment. The total number of animals is indicated. One-way ANOVA with Bonferroni’s multiple comparisons was performed. All panels: Shapiro-Wilk test was used for normality assessment (see Additional file 2). Different letters indicate significant differences, p<0.05; treatments sharing any letter are not statistically different. All graphs describe the mean ± SEM. The total number of analyzed samples is included below the corresponding panel. All datasets and statistical analysis on which the conclusions are based are included in Additional file 2