Fig. 6

Nocardia infection involved in neurological behavioral disorder. a Heatmap of the DEGs related to neurodegenerative diseases. Yes: Genes were included; No: Genes were not included. b The genes involved in neurodegenerative diseases were verified by qRT–PCR. RNA-seq, the data for dual RNA-seq. R-qRT–PCR, RNA samples from repeated experiments. PC12, RNA samples were extracted from PC12 cells infected with N. farcinica at a MOI of 10 for 6 h. c The time for the mice to turn completely downward (Tturn) and reach to the floor was recorded (n = 5 mice per group, N = 3 replicates per mouse, Student’s two-tailed T test, ***p<0.001, error bars represent SEM). d Clinical strains of Nocardia related to neurological behavioral disorder. Twenty-two clinical strains of Nocardia were analyzed, and 15 strain infections showed neurological behavioral disorders, and 7 strain infections showed few obvious neurological behavioral symptoms