Fig. 6

GTE4 regulates JA content and JA-, SA-responsive gene expression. A, B GO analysis of upregulated genes in gte4 under control (A) and Pst DC3000 treatment conditions (B). C RT-qPCR validation of JA-responsive genes expression in gte4. Data are presented as mean ± SEM of 3 technical replicates. Another biological repeat is presented in Additional file 8: Fig. S4A. D Chromatograms of LC–MS data for measurement of JA content in WT, gte4 mutants, and GTE4-HA rescue plants. XIC, extracted ion chromatograms. E Quantification of JA content in (D). Data are presented as mean ± SEM of 3 biological replicates. Student’s t-test, ***P < 0.005. F RT-qPCR of PDF1.2a/b/c and COI1 expression in gte4 and GTE4-HA plants. Data are presented as mean ± SEM of 2 biological replicates. Student’s t-test, ***P < 0.005. G RT-qPCR of PR1 and PBS3 expression in gte4 and GTE4-HA plants. Data are presented as mean ± SEM of 3 biological replicates. Student’s t-test, ***P < 0.005. H Flowering phenotype of gte4 plants. Pictures were taken at 4-week-old. I Rosetta leaf number of plants showed in (H). Data are presented as mean ± SEM of at least 30 plants