Fig. 2

Summary of evidence supporting an eccDNA forming region of interest in the M. oryzae genome. A Location of effector AvrPita3 and Mariner transposon. B Location of eccDNA forming regions. The eccDNA forming region in red was chosen for validation using outward PCR. This eccDNA forming region was considered to fully encompass AvrPita3. C Sanger sequencing read generated from outward PCR (Additional File 1: Fig. S2) that supports eccDNA forming region highlighted in red in track B. D Overall Illumina sequencing read coverage. E Junction split reads obtained from Illumina data. Split reads are joined by a dashed line. Black arrows indicate that not all reads were shown in areas with high counts. F Opposite facing read pairs obtained from Illumina data. Read pairs are joined by a solid line. Black arrows indicate that not all reads were shown in areas with high counts. G Split reads obtained from PacBio CCS data. Overlapping arrows indicate single reads mapped to the same location more than once. Split reads are joined by a dashed line. All data was obtained from a single sequenced sample (biological replicate 1, technical replicate A)