Fig. 3

Enterocyte-specific expression of Imp-L2 mediates GF-associated survival. a Neither fat body- nor muscle-specific knockdown of Imp-L2 using Cg Gal4 or Dmef Gal4, respectively, causes a significant decrease in the survival rate of GF larvae. Gut enterocyte-specific knockdown of Imp-L2 using Myo1A Gal4 causes a significant decrease in the survival rate of GF larvae. b Imp-L2 is mainly expressed in enterocytes in the larval midgut. Tiny spot-like immune signals of Imp-L2 are broadly seen in Myo1A-positive cells (enterocytes), but not in Myo1A-negative cells (arrowheads), and are more apparent in GF than in CR. c Imp-L2 is rarely expressed in esg-positive cells (intestinal stem cells/enteroblasts). GF mid-3rd instar larval guts are observed. d Imp-L2 expression, probed by immune signals (d) or enhancer trap Gal4 of Imp-L2 (e), in a subset of Prospero-positive cells (enteroendocrine cells; arrows), which is constitutive regardless of CR or GF. ** p < 0.01 (t-test). n.s., not statistically significant. The bars in the graph represent means and standard error of the mean (SEM). Each dot shows the value from each independent replicate