Fig. 1

Genome-wide distribution of histone modifications in E. diaphana and their correlation with CpG methylation. A Boxplots of mCpG, H3K27me3, H3K36me3, H3K4me3, H3K27ac, and H3K9ac peak levels in genes and repeats region of the E. diaphana genome. The solid horizontal line in each boxplot represents the median and the dotted line the mean. The solid horizontal line for each modification represents the average median of both genes and repeats (unpaired two-tailed Student’s t test; ****p < 0.0001). B Enrichment profiles of histone modifications and DNA methylation around all the protein-coding genes. x-axis is the gene locations from −2kb of TSS through gene body and +2kb of TES; y-axis is the percentage of CpG methylation (mCpG) and log enrichment of peaks for each histone modification. C Average mCpG distribution around well-positioned histone modification’s peaks. D Principal component analysis of all five histone modifications (H3K27me3, H3K36me3, H3K4me3, H3K27ac, and H3K9ac), CpG methylation (mCpG), GC content (GC %), and gene expression (exp). E Genome browser snapshot showing example distribution of all five histone modifications (H3K27me3, H3K36me3, H3K4me3, H3K27ac, and H3K9ac) in gene body and promoter of methylated; AIPGENE18521 and non-methylated; AIPGENE18575 genes. Prominent peaks are labeled with red arrows