Fig. 4
From: De novo emergence, existence, and demise of a protein-coding gene in murids
Murine D6Ertd527e protein expression and structure. a Ectopically expressed C-terminally tagged D6Ertd527e protein in NIH 3T3 and HeLa cells can be detected by Western blotting. b Expression of C-terminally HA-tagged D6Ertd527e in oocytes analyzed by immunofluorescent staining and confocal microscopy. Approximately 100 000 in vitro-transcribed mRNA molecules were microinjected into mouse fully-grown GV oocytes and the protein was visualized by immunofluorescent staining with α–HA antibody (green color). DNA was stained with DAPI (blue color). Size-bar = 20 μm. c Hypothetical folding of D6Ertd527e protein predicted by AlphaFold [43]