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Fig. 1 | BMC Biology

Fig. 1

From: Construction and characterization of a genome-scale ordered mutant collection of Bacteroides thetaiotaomicron

Fig. 1

Workflow for the construction of an ordered collection of B. theta VPI-5482 transposon mutants. Single cells from a random pool of >300,000 mutants [30] were sorted into 302 96-well plates using FACS to create a progenitor collection. After using barcode sequencing to locate strains within the progenitor collection, representative mutants for individual genes were re-arrayed to create a condensed collection of 34 96-well plates covering >2500 genes. To demonstrate the screening potential of this collection, growth and cell shape phenotypes of each strain were characterized during growth in rich medium (BHIS)

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BMC Biology

ISSN: 1741-7007

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