Fig. 3

IL1B.⁺ dSec-SC with active metabolism maintains decidual homeostasis. A UMAP map of three sub-clusters (PreSec_SC, Sec_SC, dSec_SC) of stromal cells. B The proportion of PreSec_SC, Sec_SC, and dSec_SC from different samples. C Heat map showing relative expression (z-score) of selected genes for three sub-clusters of stromal cells. D Heat map showing selected significant ligand–receptor interactions (P value < 0.05, permutation test, see “Methods”) between endometrial cells (left) and five sub-clusters of stromal cells (right). Assays were carried out at the mRNA level, but are extrapolated to protein interactions. E Circos plot of interaction network between stromal cells and other endometrial cells. F ROS levels in DSCs were detected by a 2,7‑dichlorofluorescin diacetate assay (n = 6). G The apoptosis of DSCs in control group and rh-IL1B protein (50 ng/mL) group was detected by the flow cytometry assay (n = 6). H DSCs were treated with the rh-IL1B (50 ng/mL) or vehicle for 48 h. And then the mRNA expression levels of these genes in DSCs were measured by qRT-PCR (n = 6). Data were presented as mean ± SEM and analyzed by t test. (ns, no significance; *, p < 0.05; **, p < 0.01; ***, p < 0.001)