Fig. 6
From: An IGF1-expressing endometrial stromal cell population is associated with human decidualization
EVT promotes decidualization. A Immunofluorescence staining of HLA-G and DPP4 in the decidua (n = 3). Scale bar, 20 μm (upper), 50 μm (below). B Intercellular communication analysis among all cell types in endometrium. Line color indicates ligands broadcast by the cell population of the same color. Lines connect to cell populations where cognate receptors are expressed. Line thickness is proportional to the number of ligands where cognate receptors are present in the recipient cell population. Loops indicate autocrine circuits. Map quantifies potential communication but does not account for anatomic position or boundaries of cell populations. Detailed view of ligands broadcast by extravillous trophoblasts (EVT) and other cell populations expressing cognate receptors primed to receive a signal (right). C Bubble diagram showing the average expression of decidualization-related genes of stromal cells in the Endometrium and Decidua group. D hESCs (5 × 105 cells) were co-cultured with or without HTR8/SVneo (5 × 105 cells) for 24 h. And then the mRNA expression levels of these genes in hESCs were measured by qRT-PCR (n = 6). Data were presented as mean ± SEM and analyzed by t test. (**, p < 0.01; ***, p < 0.001). E Manhattan plot indicating the Gene Ontology (GO) enrichment, and the most important GO terms of top 100 genes of EVT. Manhattan plot was drawn by online tool—g:Profile (https://biit.cs.ut.ee/gprofiler/). F Pattern diagram of EVT promoting decidualization