Fig. 2

SARS-CoV-2 infection in testicular somatic cells. a Immunofluorescence against S-protein evidencing weak labeling in peritubular myoid (pink arrowhead) and Leydig cells (green arrowhead) (scale bar =15 μm). b–c’ TEM images showing viral particles (in low and high magnification) in the Sertoli cell (SC) (scale bars = b 2 μm; b' 500 nm) and Leydig cell (LC) (scale bars = c 500 nm; c' 200 nm). d–f TEM images of non-infected Sertoli cells (SC, pink) (scale bars = e 2 μm; f–g 5μm). g 3D reconstruction of a seminiferous tubule cross-section showing non-labeled areas surrounding germ cells (red arrowheads) (Scale bar = 40 μm). h–i high magnification of non-infected Leydig cells. Arrow = tubular crest of a mitochondrion (mi) (scale bars = 500 nm). Immunofluorescence images in the testis of patient #8. TEM images in testes from patients #1, #7, and #8