Fig. 2

Flow cytometry analysis of peripheral blood lymphocyte and lymph node cells from Foxp3-EGFP rats. A Blood and B axillary lymph nodes were harvested from 12-week-old Foxp3-EGFP or wild-type (WT) rats, and single-cell suspensions were gated by SSC and FSC on lymphocytes followed by the identification with mAbs of major cell populations; TCR⁺ cells (TCR⁺CD4⁺, TCR⁺CD8⁺), TCR⁻ cells (TCR⁻CD4+ and TCR⁻CD8+), and CD161^high in total cells for NK cells. These populations were then analyzed for FOXP3 expression by EGFP expression and by using an anti-FOXP3 mAb. For both A and B, upper panels with contour plots representative from one animal and lower graphs the mean and SEM of all animals analyzed. Student’s t test *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001