Fig. 5
From: Integrative single-cell RNA-seq and ATAC-seq analysis of myogenic differentiation in pig
Cell type-specific gene regulatory landscape of pig embryonic skeletal muscle. A Heatmap showing motif enrichment analysis on the cell type-specific open chromatin regions using 10× Genomics (full results are shown in Additional file 7: Table S6). B TF expression z score heatmap that corresponding to the motif enrichment in each cell type. C Heatmap of cell type-specific regulons, as inferred by the SCENIC algorithm. Regulon activity was binarized to “on” (black) or “off” (white). D tSNE depiction of regulon activity (“on-blue”, “off-gray”), TF gene expression (red scale), and expression of predicted target genes (purple scale) of exemplary regulons for Pax3+ progenitors (MEIS1), myogenic progenitors (EZH2 and HDAC2), myoblasts (EGR1), and myocytes (MYOG). Examples of target gene expression of the TFs (PAX3, PCNA, SRSF7, RHOB, and SPG21) are shown in purple scale. Additional examples are given in Figure S6. The full list of regulons and their respective predicted target genes can be found in Additional file 11: Table S10