Fig. 5
From: Formation and characterization of BMP2/GDF5 and BMP4/GDF5 heterodimers
Type 1 and type 2 receptor affinity determined by SPR. A Relative binding constants (KD) for BMP2 (light blue), BMP4 (dark blue), GDF5 (green), BMP2/GDF5 (light purple), and BMP4/GDF5 (dark purple) to type 1 receptors Fc-(Alk3)2 and Fc-(Alk6)2, in molarity, as determined by SPR binding curves (Fig. S3) analyzed for kinetic binding using a 1:1 fit to determine association rate (ka) and dissociation rate (kd) for each ligand:receptor pair; KD=kd/ka. Reported values are the average of N=2 experiments, measured using ligand concentrations between 6.25 nM and 0.045 nM. B Binding constant (KD) for BMP2 (light blue), BMP4 (dark blue), GDF5 (green), BMP2/GDF5 (light purple), and BMP4/GDF5 (dark purple) to type 2 receptors Fc-(ActRIIa)2, Fc-(ActRIIb)2, and Fc-(BMPR2)2 in molarity, as determined by SPR binding curves (Additional file 4: Fig S4) analyzed for steady state binding. Reported values are the average of N=2 experiments, measured using ligand concentrations of 100–0.195 nM (for ActRIIa and BMPR2) or 25–0.195 nM (for ActRIIb)