Fig. 1

Methanogen profiles detected in marsupial species with 16S rRNA amplicon sequencing. The phylogenetic tree was built using the exon 28 of the von Willebrand factor (vWF) of the respective species available from the NCBI nucleotide database. MEGA-X [19] with MUSCLE was used to align the genes, and phylogeny was inferred using maximum likelihood and 1000 bootstrap replications. A The average relative abundance of respective OTUs and empty cells indicates no methanogen signal was detected. B The prevalence of respective OTUs for each species. The host diet composition is displayed as per legend, adapted from Shiffman et al. [14]. The number of samples is displayed to the right of the respective species