Fig. 3

Phylogenetic and genotypic distribution of high-quality Methanocorpusculum MAGs and isolate genomes. A Phylogenetic tree of concatenated archaeal marker gene files was produced using GTDB-Tk (v.1.3.0) [29], with Methanomicrobium mobile BP used as the outgroup. Phylogeny was inferred using FastTree (v2.1.10) [30] and visualisation by iToL (https://itol.embl.de/). MAGs and isolate genomes of ≥ 90% completeness and ≤ 5% contamination were included. Cultured isolates are identified by a red star. Bootstrap values are shown by the red (≥ 0.7) and black (≥ 0.9) circles. The black arrow denotes the common ancestor of Methanocorpusculum, the white arrow denotes the common ancestor of the Env clade and host clade 2, and the blue arrow denotes the common ancestor of the Env clade. B, C The genetic variance based on the presence/absence of KO and ortholog annotation, respectively. Gene annotations and PCA plots were generated using EnrichM (v0.4.15, https://github.com/geronimp/enrichM) with the ‘--ko’ (B) and ‘--orthologs’ (C) analyses. Genomes are coloured according to species, as per the legend