Fig. 5
Primary in vitro substrate utilisation profile of M. vombati CW153 and M. petauri MG. Strains were grown using BRN-RF10 medium without added sodium formate and sodium acetate at 37 °C with 1% (v/v) supplementation of each substrate, as listed in the legend. CO2/H2 was used for the positive control. CO2/H2 with ‘BRN+’ (i.e. with sodium formate and sodium acetate added) was used to show growth in basal BRN-RF10 medium. A, B M. vombati CW153 cultured with a headspace of CO2 and H2, respectively. C, D M. petauri MG cultured with a headspace of CO2 and H2, respectively. CO2 and H2 alone were used for negative controls. Growth was measured by optical density at 600 nm (OD600) at ~ 2 h interval