Folylpolyglutamate synthetase mRNA G-quadruplexes regulate its cell protrusion localization and enhance a cancer cell invasive phenotype upon folate repletion

Table 2 Fluorescence intensity ratios between the protruding blebs and cell body of cells in 3D Col gel

Growth condition	FPGS 3′UTR	Fluorescence intensity ratio^a bleb/cell body	p value vs. WT 3′UTR
FA-containing Col and medium	Null	0.75 ± 0.22	4.60E−12
	WT	3.91 ± 0.58
	dmGQ	N.A.
FA-free Col and medium	Null	0.58 ± 0.09	4.60E−08
	WT	4.03 ± 0.18
	dmGQ	0.25 ± 0.08	2.50E−08
FA-free Col and FA-containing medium	Null	0.29 ± 0.08	2.20E−07
	WT	3.25 ± 0.58
	dmGQ	N.A.

N.A. not applicable since no blebs were visual
^aThe fluorescence intensity ratios presented are the mean values obtained from analysis of ~10 cells ± S.D.

ISSN: 1741-7007