Table 3 Primers used in the current study
Application | Name | Sequence 5′-3′ |
|---|---|---|
3′-RACE | polyT adapter | GACGCGTGTGGACAGTCGATTTTTTTTTTTTTTTTTT |
FPGS EX11-upa | CAAAGGCATCCAGGCCAGG | |
adapter | GACGCGTGTGGACAGTCGA | |
FPGS Ex15-up | TCAGCCAAGGCCGAGACC | |
Cloning | NheI-UTR | TAAGCTAGCCACTGGCAGCCTGCACC |
UTR-HindII | AAAAAGCTTGACGCGTGTGGACAGTCG | |
MS2-Fw | ACCATGATTACGCCAAGCTTG | |
MS2-Rv | GTGAATTCGAGCTCGGTACCTC | |
overhang-BFP | CAAACAACAAGATGTGGAAGGCGGTAG | |
BFP-XhoI | CAAACTCGAGAATTAAGCTTGTGCCCC | |
SDMb | NheI-sdm | GCCATCCATGTGCTAGCCACTGGCAGCCTGCACCTG |
NheI-fix | GCCATCCATGTGCTAGTCACTGGCAGCCTGCACCTG | |
EcoRV-sdm | GGTGCCTTTTGTTTTTGGATATCCTGGTTCTGTCTAGACTGGCC | |
GQ116-sdm | GATCTAGACTGGCCTAGGTTCCATTGCTTTGTGATGTGAGGCCGGGAGAGGATGTC | |
GQ377-sdm | CCTCCCAGTGCCTTCTGTGAAGTGAGAGTGCCTCTGCCTGTGACACTGCGGGACAGAG |