Fig. 5

Distinct mPFC pyramidal neuron ensembles differently tuned to poking or reward behaviors. A Raster plot of the averaged z score at the onset (red line) of poking behavior (5 s before and 5 s after) of individual “Trigger-like” (Tri-like) and “Attention-like” (Att-like) neurons in correct (blue arrow) and incorrect (red arrow) trials, sorted by the time of maximal activities in correct trials. B Histograms of calcium events aligned to poking behavior onset (red-dotted line) of “Trigger-like” (top) and “Attention-like” (bottom) groups in correct (left) and incorrect (right) trials. C The mean z score during the Poking time window on correct, incorrect, and omission trials of the “Trigger-like” group. n = 38. D The mean z score during the Poking time window on correct, incorrect, and omission trials of the “Attention-like” group. n = 27. E Spatial distributions of “Trigger-like” and “Attention-like” neurons from the same representative mouse; scale bar, 100 μm. F Raster plot of the averaged z score at the onset (red line) of licking behaviors (5 s before and 5 s after) of individual “Reward-pursuing” (Re-pu) and “Rewarded” neurons in correct (blue arrow) and incorrect (red arrow) trials, sorted by the time of maximal activities in correct trials. G Histograms of calcium events aligned to reward onset (red-dotted line) of “Reward-pursuing” (top) and “Rewarded” (bottom) groups in correct (left) and incorrect (right) trials. H The mean z score during the reward time window on correct, incorrect, and licking trials of the “Reward-pursuing” group. n = 27. I The mean z score during the reward time window on correct, incorrect, and licking trials of the “Rewarded” group. n = 58. J Spatial distributions of “Reward-pursuing and “Rewarded” neurons from the same representative mouse; scale bar, 100 μm. K Neuronal response of each ensemble modulated by previous outcomes. Tri-like: n = 38; Att-like: n = 27; Re-pu: n = 27; Rewarded: n = 58. L Neuronal response of the “Trigger-like” ensemble modulated by previous outcomes at two parts of the low SF execution stage. n = 38. Data are represented as the mean ± SEM. ∗ p < 0.05; ∗  ∗ p < 0.01; ∗  ∗  ∗ p < 0.005; ∗  ∗  ∗  ∗ p < 0.001; n.s., not significant (p > 0.05)