Fig. 5

Altered signature of RNA modifications in mouse tissue tRNA-enriched fragments and 17–50-nt sncRNAs by hypobaric hypoxia exposure. A, D Fold changes of RNA modification levels on tRNA-enriched fragments (A) and 17–50-nt sncRNAs (D) from NC and Hypo group mouse tissues (n = 3, Additional files 10 and 11). The value > 0 indicates that the levels of modification were significantly increased under hypoxia environment, while the value < 0 means that the levels of RNA modification were significantly decreased under hypoxia exposure. B, E The comparison of RNA modification levels in tRNA-enriched fragments (B) and 17–50-nt sncRNA (E) across multiple tissues between NC and Hypo groups. C, F RNA modification level on testis tRNA-enriched fragments (C) and 17–50-nt sncRNA (F) (n = 3, Additional files 10 and 11). G, H Isolation of tRNA-enriched RNA fragments from testis (G) and liver (H) and their stability against RNase A/T1 and fetal bovine serum (FBS). The gray analysis showed the average value of three repeated experimental data that calculated by using Fluor ChemHD2. The level of tRNA in the 1640 group was considered as 1. *p < 0.05, **p < 0.01, ****p < 0.0001. Statistical analyses were conducted using Student’s t-test unpaired