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Fig. 2 | BMC Biology

Fig. 2

From: Insights into the structure-function relationship of the NorQ/NorD chaperones from Paracoccus denitrificans reveal shared principles of interacting MoxR AAA+/VWA domain proteins

Fig. 2

SDS-PAGE and Blue NativePAGE for NorQ and NorQWB. a SDS-PAGE of the purification process of NorQ. Lane 1: Cytosolic suspension. Lane 2: flow-through from Ni-NTA. Lane 3: Wash with 150–250 mM imidazole. Lane 4: Elution with a 250–500 mM imidazole gradient. b BN-PAGE of NorQ (14 μg) and NorQWB (18 μg), supplemented with 2 mM ATP and 20 mM MgCl2. The expected locations of several oligomeric states of NorQ are indicated. Original gel in Additional file 2

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BMC Biology

ISSN: 1741-7007

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