Fig. 3

SRSF1 is required for mouse primordial follicle formation. a, b Ovarian atrophy in 5 dpp cKO mice. c, d Immunostaining of VASA in 5 dpp ovaries. PF, primary follicle (cʹ, dʹ), PmF, primordial follicle (cʹʹ), cyst oocytes (dʹʹ), naked oocyte (dʹʹʹ). e Quantification of total oocytes per 5 dpp ovary. f Quantification of primordial follicles, primary follicles, and oocytes in cysts or naked per 5 dpp ovary. n = 3. g, h Ovarian atrophy in newborn cKO mice. i–l Immunostaining of VASA in newborn ovaries. kʹ, lʹ The primordial follicle and oocytes in cysts are shown in magnified views. Arrowheads, PmF; arrows, cyst oocytes. m The mRNA level of Srsf1 decreased in newborn cKO ovaries. n The mRNA level of Vasa decreased in newborn cKO ovaries. o Quantification of total oocytes per newborn ovary. Ctrl, n = 3; cKO, n = 4. p Percentages of primordial follicles and oocytes in cysts per newborn ovary. Ctrl, n = 3; cKO, n = 4. q Co-immunostaining of GM130 and VASA in newborn ovaries. r Percentage of double-positive cells in newborn ovaries. n = 2. s Srsf1 deficiency downregulated expression of various genes (e.g., Kit, Rac1, Figla, Nobox). t Co-immunostaining of KIT and VASA in newborn ovaries. u Percentage of double-positive cells in newborn cKO ovaries. n = 3. v Co-immunostaining of JAG1 and VASA in newborn ovaries. w Percentage of double-positive cells is shown in newborn cKO ovaries. n = 3. Real-time qPCR data were normalized to Gapdh (n, m) or Vasa (s). n = 3. Significance was determined by unpaired Student’s t test; detailed P value P ≥ 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001. The error bar represents the mean ± SEM. Scale bar, 20 μm (q right, t magnified, v magnified left), 50 μm (kʹ, lʹ), 100 μm (c, d), 200 μm (a, d, i–l, q left, t left, v left), 400 μm (g, h)