Fig. 4

SRSF1 is required for meiotic progression. a Volcano map displaying the distribution of differentially expressed genes from RNA-seq data. The abscissa in the figure represents the gene fold change in cKO and Ctrl mouse ovaries. |log2FoldChange|≥ 0. The ordinate indicates the significance of gene expression differences between cKO and Ctrl mouse ovaries. P ≤ 0.05. Upregulated genes are shown in red dots, and downregulated genes are shown in green dots. b Cluster heatmap of differentially expressed genes. The abscissa is the genotype, and the ordinate is the normalized FPKM (fragments per kilobase million) value of the differentially expressed gene. Red indicates a higher expression level, while green indicates a lower expression level. c Scatter plot of GO enrichment analysis of the 1271 differentially expressed genes. The 12 most effective terms were selected to draw a scatter diagram for display. d The misregulation of meiosis-related genes in 16.5 dpc cKO ovaries. Real-time qPCR data were normalized to Gapdh. The value in newborn Ctrl ovaries was set as 1.0, and the relative value in the ovaries of newborn cKO mice is indicated. n = 4. e Co-immunostaining of MSY2 and VASA in newborn Ctrl and cKO ovaries. DNA was stained with DAPI. Scale bar, 200 μm. f Percentage of double-positive cells is shown as the mean ± SEM. n = 3. g Immunostaining of SYCP3 in 5 dpp Ctrl and cKO ovaries. DNA was stained with DAPI. The dictyotene oocytes of Ctrl ovaries are shown in magnified views. Abnormal oocytes of cKO ovaries are shown in the magnified views. Scale bar, 50 μm. h Percentage of dictyotene oocytes is shown as the mean ± SEM in 5 dpp ovaries. n = 3. Significance was determined by unpaired Student’s t test; detailed P value P ≥ 0.05, *P < 0.05, ***P < 0.001, ****P < 0.0001. The error bar represents the mean ± SEM