Fig. 3

Transcriptomic analysis of the reactivation process after diapause. A Schematic diagram depicting the RNA-Seq workflow time course. Eggs were stored at low temperature for 2 days and then incubated at 37.8°C for 0.5–12 hours. The 37.8°C incubation represents the reactivation process. B PCA of overall gene expression profiles across samples in panel A. C Number of DEGs identified between blastoderms isolated from fresh oviposited eggs, low temperature-stored eggs or incubated eggs. DEGs were screened under the threshold of an FDR < 0.01 and |log2fold change| >1. The red and blue lines show upregulated and downregulated genes, respectively. The dashed line shows the number of DEGs normalized by the number of interval hours