Fig. 2

Venus reporter protein expression confirms RNA expression patterns of Tbx3. a Schematic representation of the Tbx3^+/Venus [42, 43] reporter mouse model employed to assess TBX3 expression patterns shown in c–h. Venus (VEN) expression is under the control of the Tbx3 promoter and disrupts Tbx3 expression from the knockin allele. b Schematic illustration depicting VEN expression pattern in pancreas during embryonic development and postnatal growth. c Immunofluorescence staining for NKX6-1/LAM (red/white), PDX1/CDH2 (red/white), SOX9/NKX6-1 (red/white), FOXA2/CDH2 (red/white), and Venus (VEN) (green) on E12.5 pancreata (n=3 mice). d Immunofluorescence staining for NKX6-1/COL IV (red/white), PDX1/CD49f (red/white), VIM/CDH1 (red/white), COL IV/CD49f (red/white), and VEN (green) on E15.5 pancreata (n=5 mice). e Immunofluorescence staining for pancreatic amylase (AMY)/INS (red/white), AMY/GCG (red/white), GCG/CDH2 (red/white), PECAM-1/CDH1 (red/white), and VEN (green) on E18.5 pancreata (n=4 mice). f Immunofluorescence staining for VEN (left panels) or TBX3 (right panels), both in green, in combination with CDH1 (red) on consecutive sections in E18.5 pancreata (n=1 mouse). g Immunofluorescence staining for VIM/INS (red/white), AMY/GCG (red/white), AMY/PECAM-1 (red/white), COL IV/PECAM-1 (red/white), and VEN (green) on early postnatal (P7) pancreata (n=4 mice). h Immunofluorescence staining for AMY/GCG (red/white), CDH1/CK-19 (red/white), INS/PECAM-1 (red/white), and VEN (green) on adult pancreata (n=3 mice). Cells were counterstained with DAPI. Scale bars correspond to 25 μm. n≥2 per group. E, exon