Fig. 3

Tbx3 is largely dispensable for pancreatic development. a Schematic representation of the Tbx3^fl/fl; Ptf1a^Cre/+ mouse model allowing Tbx3 deletion in pancreatic epithelial tissue (Tbx3-KO (epi)). b Hematoxylin and eosin (H&E)-stained histological sections of control and Tbx3-KO (epi) pancreata. Immunofluorescence stainings for c CK-19/AMY2A (red/green) and d INS/GCG (green/red) on control and Tbx3-KO (epi) pancreata. Quantification of e number of cells per islet based on INS/GCG co-stainings, f percentage of insulin expressing cells in islets, and g percentage of glucagon-expressing cells in Langerhans islets. n=14 control (littermates of Tbx3-KO (epi)) pancreata and n=9 Ptf1a-Cre-driven Tbx3-KO (epi) pancreata. h Schematic representation depicting Tbx3^fl/fl; Nkx3-2^Cre/+ mouse model allowing Tbx3 depletion in pancreatic mesenchyme (Tbx3-KO (mes)). i H&E-stained histological sections of control (littermates of Tbx3-KO (mes)) and Nkx3-2-Cre-driven Tbx3-KO (mes) pancreata. Immunofluorescence stainings for j CK-19/AMY2A (red/green) and k INS/GCG (green/red) on control and Tbx3-KO (mes) pancreata. Quantification of l number of cells per islet area based on INS/GCG co-stainings, m percentage of insulin expressing cells in islets, and g percentage of glucagon-expressing cells in Langerhans islets. n=7 control (littermates of Tbx3-KO (mes)) pancreata and n=4 Nkx3-2-Cre-driven Tbx3-KO (mes) pancreata. Cells were counterstained with DAPI. Scale bars correspond to 50 μm. Data are expressed as individual datapoints and mean ± SEM. Mann-Whitney test was performed to investigate significance levels (no significance reached)