Fig. 5

Genomic integrity of rehydrated P. roseola (A), Habrotrocha sp. Belgium (B), A. editae (C) after 1 day of desiccation with exposure to 800 Gy X-ray radiation and after 800 Gy X-radiation for hydrated R. macrura and R. rotatoria (D). Genomic integrity was assessed using pulsed-field gel electrophoresis (see Methods section) at different time points post irradiation (and rehydration for desiccation-resistant species). The first lane on the gels corresponds to the karyotype of Saccharomyces cerevisiae. The second lane corresponds to the control (1000 hydrated individuals, but 250 for Rotaria species). The third lane corresponds to 1-day desiccated bdelloids not submitted and submitted to 800 Gy X-ray radiation for A, B, and C, and to 250 hydrated individuals of R. macrura and R. rotatoria exposed to 800 Gy of X-ray for D. The other lanes correspond to 1000 desiccated individuals after 2, 4, 8, 24, 48, and 168 h of rehydration (A, B, C) or to 250 individuals after 2, 4, 8, 24, and 48 h post irradiation (D). The run parameters are documented under each gel