Table 2 Quality assessment of the channel catfish genome assembly Coco_2.0 and blue catfish genome assembly Billie_1.0 using International Genome 10 K (G10K) Consortium metrics [30]

Overall quality

x.y.P.Q.C

 > 6. > 30.–.39.99

 > 12. > 29.–.37.97

1. Continuity

1.1. Contig NG50 (x)

6.7 Mb

12.8 Mb

1.2. Scaffolds NG50 (y)

30.4 Mb

29.2 Mb

1.3. Gaps per Gb

170

82

2. Structural accuracy

2.1. Reliable blocks

2.8–33.9 Mb

7.2–33.6 Mb

2.2. False duplications

-

-

2.3. Curation

Manual

Manual

3. Base accuracy

1.1 Base pair QV (Q)

39.17

37.15

3.2. k-mer completeness

98.65%

98.05%

4. Haplotype phasing

1.2 Phase block NG50 (P)

-

-

5. Functional completeness

1.3 Genes

26,575

27,504

5.2. Transcript mappability

-

-

6. Chromosome status

1.4 Assigned (C)

99%

97%

1.5 Sex chromosome

X

X and Y^a

1.6 Mitochondrial genome

One complete allele, KM576102.1

One complete allele, NC_003489.1

Additional assurances

Bionano optical mapping

306X genome coverage

233X genome coverage

Additional assurances

Genetic linkage mapping

690 K SNP arrays

690 K SNP arrays