Fig. 5

A Regulation on lipid biosynthesis-related pathways. The icons next to the key enzymes represent the relative expression levels of the transcripts at 85 and 115 DAF. The heat map is displayed according to log10 [fragments per kilobase per million (FPKM) + 1] values. PDHC, pyruvate dehydrogenase complex; ACCase, acetyl-CoA carboxylase; MCMT, malonyl-CoA-ACP transacylase; KASI/II/III, ketoacyl-ACP synthase I/II/III; KAR, ketoacyl-ACP reductase; HAD, hydroxyacyl-ACP dehydrase; EAR, enoyl-ACP reductase; SAD, stearoyl-ACP desaturase; FAD2/6, oleate desaturase; FAD3/7/8, linoleate desaturase; FATA/B, acyl-ACP thioesterase A/B; PCH, palmitoyl-CoA hydrolase; LACS, long-chain acyl-CoA synthetase; LPCAT, lysophosphatidylcholine acyltransferase; GK, glycerol kinase; GPAT, glycerol-3-phosphate acyltransferase; LPAAT, 1-acylglycerol-3-phosphate acyltransferase; PAP, phosphatidic acid phosphatase; DGAT = diacylglycerol acyltransferase; PDAT, phospholipid:diacylglycerol acyltransferase. B The qRT-PCR validation of oil biosynthesis genes at two-time points of A. truncatum. The relative expression levels of genes were normalized with the internal reference gene Actin (Wang et al., 2018). Three biological replicates (nested with three technical replicates) are represented by the error bars (P < 0.05). C TF-oil biosynthesis network. Co-expressed network of the correlation among transcription factors and oil biosynthesis genes (FAD2, FAD3, and KCS). The gray lines indicate the correlations between the two genes