Fig. 4
From: Gram-negative bacteria resist antimicrobial agents by a DzrR-mediated envelope stress response
DzrR homologs from Dickeya, Ralstonia, and Burkholderia strains are highly conserved. A Sequence alignment of DzrR and its homologs from Dickeya (DzrR3937), Ralstonia (DzrRGMI1000), and Burkholderia (DzrRBGR1, DzrRJ2315, DzrRH111, and DzrR25416). B DNase I footprinting assay performed between DzrR25416 and the desAB promoter fragment (PdesAB) labeled with FAM and HEX. BSA (bovine serum albumin) was used as a negative control for analyzing the specific region protected by DzrR25416.C Sequence identity and similarity of DzrR homologs. D The transcript level of desB determined by RT-qPCR in the wild-type strain EC1, dzrR mutant, and dzrR mutant with expression of dzrR or various dzrR homologs. Fold change of desB expression was analyzed using 2−∆∆CT method, with the 16S rRNA gene serving as the endogenous control and EC1(pBB) as the reference sample. Data are presented as mean ± SE, n = 4. Statistical analysis was performed using permutation test versus ∆dzrR(pBB). *P < 0.05. E EMSA of DzrR25416 binding to the 37-bp DNA fragment (PdesAB(37)) from D. oryzae EC1. Free probe of PdesAB(37) (37 bp) and PdesAB(37) probe with DzrR homolog from B. cepacia ATCC 25416 (DzrR25416) (Bound probe) are indicated by arrows