Table 4 Plasmids used in this study
Plasmid | Comments | Reference |
|---|---|---|
pAG25 | NATMX4 | [66] |
pUG6 | KANMX | [67] |
pRRC-N | 2µ origin, natMX, pSNR52-gRNA, pROX3-cas9 | [70] |
pYES2.1 TOPO® TA | 2µ origin, pGAL1-CYC1 TT-V5 epitope-6xHis, URA3, Ampr, pUC ori, f1 ori | Invitrogen |
pYES-LacZ | pYES2.1 TOPO® TA, LacZ ORF | Invitrogen |
pYES-Sk-DGD1 | pYES2.1 TOPO® TA, DGD1(IFO1802-ChrVII) ORF | This study |
pYES-Su-DGD1 | pYES2.1 TOPO® TA, DGD1(AQ2901) ORF | This study |