Fig. 2

Global differences in the chromatin landscape between asexual parasites and gametocytes. ChIPseq was conducted on female day 6 gametocytes and ring stage parasites using antibodies against H3K4me3 (n = 2 for female gametocytes and ring stage parasites), H3R17me2 (n = 2 for female gametocytes and ring stage parasites), H3K27ac (n = 3 for female gametocytes, n = 2 for ring stage parasites), H2A.Z (n = 6 for female gametocytes, n = 2 for ring stage parasites), and H3K9me3 (n = 5 for female gametocytes, n = 2 for ring stage parasites). A Overview of log₂-transformed ChIP/Input ratio tracks from female day 6 gametocytes and ring stage parasites along chromosome 2. Genes are indicated at the bottom, with green and orange referring to the transcriptional orientation. B Line plots showing the average log₂-transformed ChIP/Input coverage of genes (ATG to STOP) and their 2 kb up- and downstream regions for each modification in female gametocytes (upper panel) and rings (lower panel) sorted into groups according to expression levels from stage-matched RNAseq data. Heterochromatic genes were defined by H3K9me3 enrichment 500 bp around the ATG. Silent genes were defined as genes with 0 FPKM by RNA-Seq. The remaining genes were divided into top, medium and bottom transcribed tertiles