Fig. 5

H2A.Z is associated with heterochromatic areas in day 6 female gametocytes. A Overview over chromosome 2 showing log₂-transformed ChIP/Input coverage for H2A.Z (deep blue) (n = 6 for female gametocytes, n = 2 for male gametocytes, n = 2 for ring stage parasites) and H3K9me3 (deep red) (n = 5 for female gametocytes, n = 2 for male gametocytes, n = 2 for ring stage parasites) in rings, male and female gametocytes as well as H2B.Z (purple) in female gametocytes (n = 1). Lower panel shows zoom into the heterochromatin/euchromatin boundary in the left subtelomeric region of chromosome 2, which includes several var, stevor (marked with an “s”), and rif genes. Genes are marked by orientation either green (+ genes) or orange (- genes), with introns marked in either light green or dark red. B Average profile plots of log₂-transformed ChIP/Input signals for H2A.Z and H2A.Zac over gene bodies (ATG to STOP) and their 2 kb up-/downstream region in male and female day 6 gametocytes grouped by gene expression. C Correlation scatter plot of log₂-tranformed ChIP/Input H2A.Z coverage per 150 bp bin comparing the different parasite stages against each other. R², spearman correlation. D Correlation scatter plot of H2A.Z versus H2B.Z in female day 6 gametocytes