Fig. 4
From: N-terminal region of Drosophila melanogaster Argonaute2 forms amyloid-like aggregates
Fluorescence imaging of the Nter aggregate morphology. A–C Representative fluorescence images of linear fractal-shaped (A), branched fractal-shaped (B), and fibril-shaped aggregates (C). Fluorescence signals from mCherry (left panels, magenta) and ThT (middle panels, green) showed almost identical images. Merged images are shown in the right panels. Scale bars represent 1 μm (A, B) and 5 μm (C). D Fluorescence intensity profiles of mCherry and ThT along the line in the merged image of (C). Intensities were normalized. E PCA analysis of the shapes of the Nter aggregates. The plots were colored according to the classified group (blue, linear fractal-shaped; green, branched fractal-shaped; orange, fibril-shaped)